Low budget low-tech human organ factory

Low budget low-Tech human organ factory;

Any Atheists with  big purses  if you want  to support financially I am willing to  gather  a willing team to try this  practically.

Before starting this thought experiment and my hypothesis, I want to make the  following statements.

1)   I am an atheist, I was brought up as a Hindu and when I was a child believed in reincarnation and that all living beings including plants and insects have an “atman “ (loosely translated to soul). So I don't give a rat’s arse what the religious believers and ethicists think about my experiment.

2)   I think all the restrictions being placed on human cloning by different governments is stupid.

3)   When it is ok to clone one animal then it is  ok to clone  another .Humans  are nothing more than a  species in the animal kingdom.

4)   If we get over the initial “ yikes” factor we can have an unlimited supply of human organs to help people live longer and healthier lives.

What do we need?

A human who needs a new heart. He is willing to spend time and money to grow a new heart.

A woman in the reproductive age who is willing to donate few of her ova ( If  she  is  maternally related to  the  patient it is very good , this solves the  mitochondrial DNA  problems . if  she is  also HLA compatible  it is even better.

An IVF lab

A few dozen Emu /Dromaius novaehollandiae eggs

Incubators /microscopes tissue culture media / dissection instruments /electroproation equipment/laser shot equipment

A transplant surgeon with his /her team.

Any one who wants to really duplicate this thought experiment in to real world practical experiment should  read  the  following  papers  first .

http://www.cdb.riken.jp/eeg/_userdata/DD_emu.pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3157849/

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Daniel S. Dohle, Susanne D. Pasa, Sebastian Gustmann, Markus Laub, Josef H. Wissler, Herbert P. Jennissen, and Nicole Dünker

this shows how  a  mouse limb grows and  differentiates  in  a petri dish ex ovo chick culture

Step 1

 Collect patient’s semen

Collect a skin biopsy and set up a fibroblast culture

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392772/

Production and validation of a good manufacturing practice grade human fibroblast line for supporting human embryonic stem cell derivation and culture

Nilendran Prathalingam,#1,2 Linda Ferguson,#1,3 Lesley Young,4 Georg Lietz,5 Rachel Oldershaw,1,3 Lyn Healy,4 Albert Craig,1,6 Helen Lister,1,6 Rakesh Binaykia,1 Radhika Sheth,1 Alison Murdoch,1,6,7 and Mary Herbert1,2,7

 Use one of the well known ovarian stimulation protocols and using good clinical practice collect as many ova as you can.

Take half these ova and perform ICSI on them using the patient’s semen .

Enucleate the other half ova and perform SCNT and electroproation to create human clones.

Setup ex ovo culture using emu eggs in plastic containers and in their original shells with  a window  cut in to them.

When the human IVF/ICSI/SCNT embryos are 6 to 8 cell stage transplant them on to the Emu chorioallantoic membrane (CAM) 

Decapitate the emu embryo using the laser shot.

Let the human embryo incubate in this in ovo /ex ovo culture for as many days as possible. One can setup some way to slowly keep supplementing   yolk to the culture from other emu eggs.

 Operate on the   organ recipient, isolate a small portion of the colon and exteriorize it as a isolated intestinal pouch.

After decapitating and stripping it of limbs and other extra organs transfer the growing heart and other organs on to the exterioirized intestinal sac.

If the development of the embryo is good and a micro vascular anastamosis can be performed it is even better even otherwise the developing chorionic villi will  burrow in  to the  intestinal  pouch.( as shown in primary and  secondary abdominal pregnancies)After 2 to 3 months you should have organs viable and big enough to transplant as piggybacks to recipient’s own organs and after a year or two if needed his original dysfunctional organs can be excised and the new organs  fixed  orthotopically.

 All this can be achieved with existing technology using basic medical and lab equipment available off the shelf.

 I have used a male recipient because SCNT human embryo viability is still not proven in culture conditions. Where as there is nothing to prevent a regular IVF/ICSI embryo from growing in an ex ovo emu culture. When mouse limb buds can grow, human hearts and other organs should also grow and mature.