Low budget low-tech human organ factory

Low budget low-Tech human organ factory;

Any Atheists with  big purses  if you want  to support financially I am willing to  gather  a willing team to try this  practically.

Before starting this thought experiment and my hypothesis, I want to make the  following statements.

1)   I am an atheist, I was brought up as a Hindu and when I was a child believed in reincarnation and that all living beings including plants and insects have an “atman “ (loosely translated to soul). So I don't give a rat’s arse what the religious believers and ethicists think about my experiment.

2)   I think all the restrictions being placed on human cloning by different governments is stupid.

3)   When it is ok to clone one animal then it is  ok to clone  another .Humans  are nothing more than a  species in the animal kingdom.

4)   If we get over the initial “ yikes” factor we can have an unlimited supply of human organs to help people live longer and healthier lives.

What do we need?

A human who needs a new heart. He is willing to spend time and money to grow a new heart.

A woman in the reproductive age who is willing to donate few of her ova ( If  she  is  maternally related to  the  patient it is very good , this solves the  mitochondrial DNA  problems . if  she is  also HLA compatible  it is even better.

An IVF lab

A few dozen Emu /Dromaius novaehollandiae eggs

Incubators /microscopes tissue culture media / dissection instruments /electroproation equipment/laser shot equipment

A transplant surgeon with his /her team.

Any one who wants to really duplicate this thought experiment in to real world practical experiment should  read  the  following  papers  first .

http://www.cdb.riken.jp/eeg/_userdata/DD_emu.pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3157849/

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Daniel S. Dohle, Susanne D. Pasa, Sebastian Gustmann, Markus Laub, Josef H. Wissler, Herbert P. Jennissen, and Nicole Dünker

this shows how  a  mouse limb grows and  differentiates  in  a petri dish ex ovo chick culture

Step 1

 Collect patient’s semen

Collect a skin biopsy and set up a fibroblast culture

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392772/

Production and validation of a good manufacturing practice grade human fibroblast line for supporting human embryonic stem cell derivation and culture

Nilendran Prathalingam,#1,2 Linda Ferguson,#1,3 Lesley Young,4 Georg Lietz,5 Rachel Oldershaw,1,3 Lyn Healy,4 Albert Craig,1,6 Helen Lister,1,6 Rakesh Binaykia,1 Radhika Sheth,1 Alison Murdoch,1,6,7 and Mary Herbert1,2,7

 Use one of the well known ovarian stimulation protocols and using good clinical practice collect as many ova as you can.

Take half these ova and perform ICSI on them using the patient’s semen .

Enucleate the other half ova and perform SCNT and electroproation to create human clones.

Setup ex ovo culture using emu eggs in plastic containers and in their original shells with  a window  cut in to them.

When the human IVF/ICSI/SCNT embryos are 6 to 8 cell stage transplant them on to the Emu chorioallantoic membrane (CAM) 

Decapitate the emu embryo using the laser shot.

Let the human embryo incubate in this in ovo /ex ovo culture for as many days as possible. One can setup some way to slowly keep supplementing   yolk to the culture from other emu eggs.

 Operate on the   organ recipient, isolate a small portion of the colon and exteriorize it as a isolated intestinal pouch.

After decapitating and stripping it of limbs and other extra organs transfer the growing heart and other organs on to the exterioirized intestinal sac.

If the development of the embryo is good and a micro vascular anastamosis can be performed it is even better even otherwise the developing chorionic villi will  burrow in  to the  intestinal  pouch.( as shown in primary and  secondary abdominal pregnancies)After 2 to 3 months you should have organs viable and big enough to transplant as piggybacks to recipient’s own organs and after a year or two if needed his original dysfunctional organs can be excised and the new organs  fixed  orthotopically.

 All this can be achieved with existing technology using basic medical and lab equipment available off the shelf.

 I have used a male recipient because SCNT human embryo viability is still not proven in culture conditions. Where as there is nothing to prevent a regular IVF/ICSI embryo from growing in an ex ovo emu culture. When mouse limb buds can grow, human hearts and other organs should also grow and mature.

where evil lurks

This is exactly what  was happening to me  in the  VA

with the  socalled   PACT (patient aligned care team/started by this lady from  Duke  by the name of  Gaudet)

Where evil lurks

They live in the basement, back in a dark, damp corner.  Thats them with their laptop, sitting on their cot, conniving against us.  They exist in the shadows, never coming out into the light. They slink around the hospital... unseen but always looming, always waiting, ready to pounce. Who are they?

They are the people who make your life a living hell.   The people who MAKE THE CHANGES. (ThE EVIL cHanGerS)  They are the ones who decide to buy the stupid new products because they get a good deal.   They change the electronic charting to "make it better".  They come up with irrelevant new policies.

Of course ThE EVIL cHanGerS make twice the money you do. They haven't seen a bedside in 20 years. They have a lot of letters after their name. They wear REGULAR clothes.  They like to gather in groups to scheme about new changes. They seem to breed like rabbits.

They buy things like the new .5 mg Dilaudid syringes which take a rocket scientist to figure out how to use and actually come with a video to explain it. They buy IV tubing in which connecting tubing to a saline lock or connecting a piggyback requires 20 attempts. They change charting to "become more efficient".  The thing is it requires 20 more steps to complete. It makes charting a blood transfusion akin to building a nuclear reactor.

They give us more gray hair. Make us want to go running screaming from the building.  Want to wring somebodys neck.  Make us spend the majority of our time away from the patients filling in blanks.

They are  ***ThE EVIL cHanGerS****.  Some day the nurses will rise up.  They will hunt them down and there will be hell to pay.


excerpted from the blog /http://emergency-room-nurse.blogspot.com/

madness: tales of an emergency room nurse

Indian language computer applications an appeal

Indian language computer applications an appeal

Dear

 Everyone who cares,

It is sad that with a large software workforce, India is still lagging behind in improving the representation of Indian  regional languages  in various computer software programs dealing with   language in general.

I am a physician from Andhra Pradesh presently in Dallas TX .I am interested in Telugu language computer applications.

I had raised the topic of creating a telugu OCR software in a discussion list called "Racchabanda" in 1996 and again in 1998. as recently as   2010 we were still discussing  about this  in Google group called " telugu sanganana"

 Some guy might come up and tell me this OCR problem is already solved, by some software from either TIDl, IIIT or University of Hyderabad.

But unfortunately after claiming to have produced a software and stating there is 95% accuracy these software programs disappear.

There is not a single freeware or commercial program which does the following for Telugu with ease and accuracy.( like  MS  word/Omni page/Dragon naturally speaking for English)

spellcheck, OCR, speech recognition .handwriting recognition, machine translation( Google translate is a joke ) speech synthesis( this is to a certain extent solved,  but much can be done to improve it)

The status is similar for most other Indian languages.

I was almost given up on this but the recent success of AAM AADMI Party has energized me and I think  a handful of people can really make a difference , if they  concentrate and  strive.

So I am   inviting all of you to come forward and spend a few hours each week to develop the following for telugu and  this will in turn encourage  others to develop similar  projects for  all the Indian languages.

I made a beginning by sending  an email to the professor  who developed the  search engine yioop and got the  following  reply.  This is  encouraging.

FIRST we need a large public corpus.

Second we need  to tag the  corpus

Third  we need to complete the  boot strapping of tesseract  for OCR.

After  succeeding at these, we can go on to new projects.

who wants to volunteer?

Me

To chris@pollett.org

Dec 12 at 9:43 PM

Hi,

I am a physician  fro  Ap  presently in Dallas TX  .I am interested in Telugu language  computer applications.

I am  interested in . OCR, MT, speech and  handwriting recognition.

I am not  very good at  computer programming but have  a wider understanding .

there is no large  corpus  publicly available  for this INDIC  language.

I was trying to create one  and  started using  web as corpus   software but unfortunately due to  the  loss of  BING API this is no longer working.

can  the  crawler  you use  be used to create such a corpus ?

I would like to interact with you on some ideas I have .

are you willing to correspond with me on email

Chris Pollett

To Me

Dec 12 at 10:57 PM

Sure. I don't know Telugu, but I could put you in contact with some former students who worked on Yioop

that do. Looking at the most recent crawl I did, I did get a fair number of Telugu documents

but they were mainly Wikipedia related:

http://www.yioop.com/index.php?q=lang%3Ate&YIOOP_TOKEN=xlhhSDmlPjI|1386909727&its=1375222073&limit=0

Restricting to both the telugu language and .in domain yields only a small number of

results in my last crawl:

http://www.yioop.com/index.php?YIOOP_TOKEN=gTXbgBRIWgI|1386909940&its=1375222073&q=lang%3Ate+site%3Ain

all of which seem to be mainly English. My crawl on the machines in our guest room is only about 1/100th the size of Bing

or Google's index and is mainly of American sites, but you can configure Yioop to crawl whatever you like. If you 

downloaded the Telugu Wikipedia (available free), Yioop could index it. If you had a good start list of URLs it would possible to have Yioop 

crawl and only index the sites it found containing Telugu.

Best,

Chris 

amithkc@gmail.com,
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priyaverma123@imsec.ac.in,
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SAMBHA.GUPTA@GMAIL.COM,
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3181993vivek@gmail.com,
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NIRUPAMTIEARI101093@GMAIL.COM,
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