The Genetic Network of Life-Span Extension by Dietary Restriction

DR Regimens in C. elegans
C. elegans normally live in the soil and feed on bacteria,for example, those present on rotten fruits. In the laboratory, worms are traditionally grown on a thin film of Escherichia coli bacteria spread on solid agar plates. Twelve DR regimens have been developed in C. elegans, and they all extend life span, albeit to different degrees


Twelve methods of dietary restriction in C. elegans

Method	MedIuM	Food source	aGe InItIated	FertILItY	%Increasea	reFerences
Axenic	Synthetic	Defined	Larval day	Decrease	50–150%	[1–5]
CDLM	Synthetic liquid	Defined chemical broth	Birth	Decrease	88%	[6]
BDR	Liquid	Live E. coli	Day 2 of	Decrease	32–101%	[5,7–11]
		(antibiotics)	adulthood
LDR	Liquid 	Live E. coli	L4/young	Decrease	28%	[12]
	solid	(antibiotics)	adult
DP	Solid	Live E. coli	Birth	Increase	25–33%	[10,13]
sDR	Solid	Live or dead	Day 4 of	Decrease	18–35%	[10,14,15]
		E. coli	adulthood
sDR(H)	Solid	Live E. coli	Day 2 of	Decrease	13–18%	[16]
			adulthood
msDR	Solid	Live E. coli	Day 1 of	Decrease	35–47%	[17]
		(antibiotics)	adulthood
sDR(C)	Solid	Live E. coli	Day 5 of	NT	23–28%	[11]

Different laboratories calculate life span starting at different ages (birth vs young adult), which affects the total % increase.
b[1] Vanfleteren & Braeckman, 1999; [2] Houthoofd et al., 2002; [3] Houthoofd et al., 2002; [4] Houthoofd et al., 2003; [5] Zhang
et al., 2009; [6] Szewczyk et al., 2006; [7] Klass, 1977; [8] Johnson, 1990; [9] Panowski et al., 2007; [10] Greer & Brunet, 2009;
[11] Carrano et al., 2009; [12] Bishop & Guarente, 2007; [13] Hosono et al., 1989; [14] Greer et al., 2007; [15] Park et al., 2009a;
[16] Honjoh et al., 2009; [17] Chen et al., 2009; [18] T. L. Kaeberlein et al., 2006; [19] Lee et al., 2006; [20] Steinkraus et al., 2008;
[21] Smith et al., 2008; [22] Mehta et al., 2009; [23] Lakowski & Hekimi, 1998; [24] Wang & Tissenbaum, 2006; [25] Curtis et al.,
2006; [26] Henderson et al., 2006; [27] Hansen et al., 2005; [28] Hansen et al., 2007; [29] Hansen et al., 2008; [30] Iser & Wolkow,
2007; [31] Jia & Levine, 2007; [32] Hsu et al., 2003.