Wednesday, March 21, 2018

Shotgun sequencing −The Slurry with the Fringe on the Top

It's kind of like putting together a jigsaw puzzle with millions of pieces 
(a lot of which are “blue sky”).


Collect a sample of your cells by rinsing out your mouth with dilute salt water. Add some detergent to dissolve the cell and nuclear membranes, releasing the contents. Precipitate out the proteins by adding alcohol; stir, and let settle. In a few minutes, fibres will rise out of the murk to the top of the vessel. These fibres are your DNA (plus that of some bacteria that were living in your mouth). The sequence of your DNA is your lifelong endowment. It determined that you are human, that you are male or female, that you are brown- or blue-eyed, that you are right- or left-handed. But think of these fragile cables not as a leash that constrains you but as the cords that raise the curtains on the drama of your life. −The Slurry with the Fringe on the Top

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